Histrap ff manual

HisTrap FF crude Kit1 3 × 1 ml columns, premade buffers, connectors, syringe His Buffer Kit2 1 kit 1 Contains 3 × 1 ml HisTrap FF crude columns, premade sodium phosphate buffer concentrate (2 × 50 ml phosphate buffer, pH ), 2 M imidazole pH , connectors, syringe, and instructions. HisTrap™ FF crude Kit is designed for rapid and convenient manual purification of histidine-tagged proteins using premade buffers and a syringe. Histidine-tagged proteins can be purified directly from unclarified cell lysates. This saves time . HisTrap FF. HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). Change www.doorway.ruted Reading Time: 9 mins.

• HisTrap™ excel, 1 ml and 5 ml columns prepacked with Ni Sepharose excel. The special type of filters in the top and bottom of HisTrap excel columns makes it possible to directly load cell-free, unclarified samples on the columns without causing back pressure problems. The HisTrap excel columns can be operated with a peristaltic. Instructions AF HisTrap affinity columns GE Healthcare HisTrap HP, 1 ml and 5 ml HisTrap™ HP is a ready to use column, prepacked with precharged Ni Sepharose™ High Performance. This prepacked column is ideal for preparative purification of Histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).

31 thg 7, available prepacked in 1 and 5-ml HisTrap™ FF, 1 and 5-ml. HisTrap FF crude and ml HisPrep™ FF 16/10 columns. Ni Sepharose 6 Fast Flow has low nickel ion (Ni2+) leakage and is compatible with a wide range of additives used in protein purification. HisTrap FF columns can. media and prepacked columns permit manual purification with a syringe, a centrifuge, or by gravity-flow, HisTrap FF crude. 1 × 1 ml Approx.